Access

Research

Nature Biotechnology 25, 1483–1487 (1 December 2007) | doi:10.1038/nbt1355

Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes

Marta Fern|[aacute]|ndez-Su|[aacute]|rez , Hemanta Baruah , Laura Mart|[iacute]|nez-Hern|[aacute]|ndez , Kathleen T Xie , Jeremy M Baskin , Carolyn R Bertozzi & Alice Y Ting

Live cell imaging is a powerful method to study protein dynamics at the cell surface, but conventional imaging probes are bulky, or interfere with protein function, or dissociate from proteins after internalization. Here, we report technology for covalent, specific tagging of cellular proteins with chemical probes.