Article abstract
Nature Biotechnology , (2007)
Published online: 25 November 2007 | Corrected online: 8 July 2008 | doi:10.1038/nbt1365
There is a Corrigendum (July 2008) associated with this Article.
Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells
Gabsang Lee1, Hyesoo Kim1,4, Yechiel Elkabetz1,4, George Al Shamy2, Georgia Panagiotakos2, Tiziano Barberi3, Viviane Tabar2 & Lorenz Studer1,2
Abstract
Vertebrate neural crest development depends on pluripotent, migratory precursor cells. Although avian and murine neural crest stem (NCS) cells have been identified, the isolation of human NCS cells has remained elusive. Here we report the derivation of NCS cells from human embryonic stem cells at the neural rosette stage. We show that NCS cells plated at clonal density give rise to multiple neural crest lineages. The human NCS cells can be propagated in vitro and directed toward peripheral nervous system lineages (peripheral neurons, Schwann cells) and mesenchymal lineages (smooth muscle, adipogenic, osteogenic and chondrogenic cells). Transplantation of human NCS cells into the developing chick embryo and adult mouse hosts demonstrates survival, migration and differentiation compatible with neural crest identity. The availability of unlimited numbers of human NCS cells offers new opportunities for studies of neural crest development and for efforts to model and treat neural crest–related disorders.
- Developmental Biology Program, Sloan-Kettering Institute, 1275 York Ave., New York, New York 10021, USA.
- Department of Neurosurgery, Sloan-Kettering Institute, 1275 York Ave., New York, New York 10021, USA.
- Division of Neuroscience, Beckman Research Institute of The City of Hope, 1500 E. Duarte Road, Duarte, California 91010, USA.
- These authors contributed equally to this work.
Correspondence to: Lorenz Studer1,2 e-mail: studerl@mskcc.org
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