Letter abstract
Nature Biotechnology 25, 1177 - 1181 (2007)
Published online: 27 August 2007 | doi:10.1038/nbt1335
Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells
Alexander Meissner1,3, Marius Wernig1,3 & Rudolf Jaenisch1,2
In vitro reprogramming of somatic cells into a pluripotent embryonic stem cell–like state has been achieved through retroviral transduction of murine fibroblasts with Oct4, Sox2, c-myc and Klf4 (refs. 1, 2, 3, 4). In these experiments, the rare 'induced pluripotent stem' (iPS) cells were isolated by stringent selection for activation of a neomycin-resistance gene inserted into the endogenous Oct4 (also known as Pou5f1) or Nanog loci2, 3, 4. Direct isolation of pluripotent cells from cultured somatic cells is of potential therapeutic interest, but translation to human systems would be hindered by the requirement for transgenic donors in the present iPS isolation protocol. Here we demonstrate that reprogrammed pluripotent cells can be isolated from genetically unmodified somatic donor cells solely based upon morphological criteria.
- Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA.
- Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge Massachusetts 02142, USA.
- These authors contributed equally to this work.
Correspondence to: Rudolf Jaenisch1,2 e-mail: jaenisch@wi.mit.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Turning skin into embryonic stem cellsNature Medicine News and Views (01 Jul 2007)
Stem cells The magic brewNature News and Views (19 Jul 2007)
See all 12 matches for News And ViewsRESEARCH
In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like stateNature Article (19 Jul 2007)
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2Nature Biotechnology Research (01 Nov 2008)
See all 34 matches for Research
