Figure 2 - Clonal human AFS cells are broadly multipotent.
From the following article
Isolation of amniotic stem cell lines with potential for therapy
Paolo De Coppi, Georg Bartsch, Jr, M Minhaj Siddiqui, Tao Xu, Cesar C Santos, Laura Perin, Gustavo Mostoslavsky, Angéline C Serre, Evan Y Snyder, James J Yoo, Mark E Furth, Shay Soker & Anthony Atala
Nature Biotechnology 25, 100 - 106 (2007) Published online: 7 January 2007
doi:10.1038/nbt1274
Clonality of AFS cells and differentiated cells derived from was verified by retroviral marking. (a) RT-PCR analysis of mRNAs for lineages indicated using a retrovirally marked second round subclone of AFS cells. U: Control undifferentiated cells. D: cells maintained under conditions to promote osteogenic (8 d), myogenic (8 d), adipogenic (16 d), endothelial (8 d), hepatic (45 d), neurogenic (8 d) differentiation. (b) Southern blot analysis of inserted GFP retroviral DNA in differentiated cells from (a); arrow indicates 4-kbp junction fragment in BamH1 digested DNA from undifferentiated cells of a GFP positive subclone of AFS cells (lane 1) and the second round subclone (lane 2) used for transcript analysis after differentiation under adipogenic (lane 3), endothelial (lane 4), hepatic (lane 5), osteogenic (lane 6), myogenic (lane 7), and neurogenic (lane 8) conditions.
