Nature Biotechnology
- 24, 1115 - 1122 (2006)
Published online: 8 September 2006; | doi:10.1038/nbt1236
Evaluation of DNA microarray results with quantitative gene expression platformsRoger D Canales, Yuling Luo, James C Willey, Bradley Austermiller, Catalin C Barbacioru, Cecilie Boysen, Kathryn Hunkapiller, Roderick V Jensen, Charles R Knight, Kathleen Y Lee, Yunqing Ma, Botoul Maqsodi, Adam Papallo, Elizabeth Herness Peters, Karen Poulter, Patricia L Ruppel, Raymond R Samaha, Leming Shi, Wen Yang, Lu Zhang & Federico M Goodsaid Supplementary Fig. 1 (doc 30K)
TaqMan® assays SD vs. CT plot. Supplementary Fig. 2 (doc 170K)
TaqMan® assays R2 distribution plot. Supplementary Fig. 3 (doc 45K)
Boxplot of fold change as a function of signal. Supplementary Fig. 4 (doc 90K)
Expression characteristics of EPHA7. Supplementary Fig. 5 (doc 205K)
Endogenous control expression. Supplementary Table 1 (doc 50K)
Gene lists used for the analysis of performance metrics of alternative quantitative platforms and for comparison with microarrays. Supplementary Table 2 (doc 160K)
Inter-site discordance in detection among genes expressed at low level. Supplementary Table 3 (doc 100K)
TPR, FDR tables. Supplementary Table 4 (doc 40K)
Discordant probes among the 997 genes. Supplementary Table 5 (doc 45K)
Discordant gene expression values in alternative quantitative platforms FC expression values for all discordant genes. Supplementary Methods (doc 50K)
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