Nature Biotechnology 24, 820 - 821 (2006)
Published online: 25 June 2006; | doi:10.1038/nbt1221
Transposon-free insertions for insect genetic engineeringTarig H Dafa'alla1, 3, George C Condon1, 3, Kirsty C Condon1, 2, Caroline E Phillips1, Neil I Morrison1, 2, Li Jin1, Matthew J Epton2, Guoliang Fu1
& Luke Alphey1, 2, 31
Oxitec Limited, 71 Milton Park, Oxford
OX14 4RX, UK. 2
Department of Zoology, University of Oxford, South Parks Road, Oxford
OX1 3PS, UK. 3
These authors contributed equally to this work.
Correspondence should be addressed to Luke Alphey luke.alphey@zoo.ox.ac.uk Methods involving the release of transgenic insects in the field hold great promise for controlling vector-borne diseases and agricultural pests. Insect transformation depends on nonautonomous transposable elements as gene vectors. The resulting insertions are stable in the absence of suitable transposase, however, such absence cannot always be guaranteed. We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity.
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