Journal home
Advance online publication
Current issue
Archive
Press releases
Supplements
Focuses
Conferences
Guide to authors
Online submissionOnline submission
Permissions
For referees
Free online issue
Contact the journal
Subscribe
Advertising
work@npg
naturereprints
About this site
For librarians
Research
Nature Biotechnology 24, 703 - 707 (2006)
Published online: 14 May 2006; | doi:10.1038/nbt1210

A microengraving method for rapid selection of single cells producing antigen-specific antibodies

J Christopher Love, Jehnna L Ronan, Gijsbert M Grotenbreg, Annemarthe G van der Veen & Hidde L Ploegh

Supplementary Fig. 1 (doc 172K)
Analysis of efficiency for loading microwells with cells.

Supplementary Fig. 2 (doc 28K)
Measurement of the viability of individual cells in a closed microenvironment over time.

Supplementary Fig. 3 (doc 408K)
Fluorescence micrographs of two microarrays of anti-H-2Kb (red) and anti-ovalbumin (green) prepared sequentially using the same array of microwells loaded with Y3 and Hyb 099-01 cells.

Supplementary Fig. 4 (doc 388K)
Fluorescence micrographs of the regions from which the images shown in Figure 3 of the manuscript are taken.


 Top
SUPPLEMENTARY INFO
Back to article
Table of contents
Download plugins

naturejobs

natureproducts

Search buyers guide:

 
Nature Biotechnology
ISSN: 1087-0156
EISSN: 1546-1696
Journal home | Advance online publication | Current issue | Archive | Press releases | Supplements | Focuses | Conferences | For authors | Online submission | Permissions | For referees | Free online issue | About the journal | Contact the journal | Subscribe | Advertising | work@npg | naturereprints | About this site | For librarians
Nature Publishing Group, publisher of Nature, and other science journals and reference works©2006 Nature Publishing Group | Privacy policy