Nature Biotechnology 24, 703 - 707 (2006)
Published online: 14 May 2006; | doi:10.1038/nbt1210
A microengraving method for rapid selection of single cells producing antigen-specific antibodiesJ Christopher Love, Jehnna L Ronan, Gijsbert M Grotenbreg, Annemarthe G van der Veen
& Hidde L Ploegh Supplementary Fig. 1 (doc 172K) Analysis of efficiency for loading microwells with cells. Supplementary Fig. 2 (doc 28K) Measurement of the viability of individual cells in a closed microenvironment over time. Supplementary Fig. 3 (doc 408K) Fluorescence micrographs of two microarrays of anti-H-2Kb (red) and anti-ovalbumin (green) prepared sequentially using the same array of microwells loaded with Y3 and Hyb 099-01 cells. Supplementary Fig. 4 (doc 388K) Fluorescence micrographs of the regions from which the images shown in Figure 3 of the manuscript are taken.
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