 | Figure 2
Nature Biotechnology 24, 713 - 717 (2006)
Published online: 4 June 2006; | doi:10.1038/nbt1213
Herbicide-resistance conferred by expression of a catalytic antibody in Arabidopsis thalianaYael Weiss, Avidor Shulman, Irina Ben Shir, Ehud Keinan
& Shmuel Wolf | | | | Figure 2. In vitro retroaldolase activity of 38C2 (Fab) extracted from E. coli.
Methodol (compound 10, 50  M) was added to three dialyzed solutions, 25 l each, containing: protein extract of light chain (LC)-producing bacteria; protein extract of heavy chain (HC)-producing bacteria; and a dialyzed mixture of both chains (HC&LC). The mixtures were incubated overnight at 30 °C and then tested for fluorescence (irradiation at 320 nm, emission at 452 nm). The figure shows data from three independent experiments of each mixture placed as a droplet on a glass plate. C, control; LC, light chain–producing bacteria; HC, heavy chain–producing bacteria; HC&LC, dialyzed mixture of both chains.
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