Nature Biotechnology 24, 673 - 679 (2006)
Published online: 14 May 2006; | doi:10.1038/nbt1212
Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophilaNicolas Vodovar1, David Vallenet2, Stéphane Cruveiller2, Zoé Rouy2, Valérie Barbe2, Carlos Acosta1, Laurence Cattolico2, Claire Jubin2, Aurélie Lajus2, Béatrice Segurens2, Benoît Vacherie2, Patrick Wincker2, Jean Weissenbach2, Bruno Lemaitre1, Claudine Médigue2
& Frédéric Boccard11
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France. 2
Genoscope, Centre National de Séquençage and CNRS-UMR8030, 2 rue Gaston Crémieux, 91057 Evry Cedex, France.
Correspondence should be addressed to Frédéric Boccard boccard@cgm.cnrs-gif.fr CT573326
Pseudomonas entomophila is an entomopathogenic bacterium that, upon ingestion, kills Drosophila melanogaster as well as insects from different orders. The complete sequence of the 5.9-Mb genome was determined and compared to the sequenced genomes of four Pseudomonas species. P. entomophila possesses most of the catabolic genes of the closely related strain P. putida KT2440, revealing its metabolically versatile properties and its soil lifestyle. Several features that probably contribute to its entomopathogenic properties were disclosed. Unexpectedly for an animal pathogen, P. entomophila is devoid of a type III secretion system and associated toxins but rather relies on a number of potential virulence factors such as insecticidal toxins, proteases, putative hemolysins, hydrogen cyanide and novel secondary metabolites to infect and kill insects. Genome-wide random mutagenesis revealed the major role of the two-component system GacS/GacA that regulates most of the potential virulence factors identified.
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