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Nature Biotechnology 24, 572–576 (1 June 2006) | doi:10.1038/nbt1202

Preventing gene silencing with human replicators

Haiqing Fu , Lixin Wang , Chii-Mei Lin , Sumegha Singhania , Eric E Bouhassira & Mirit I Aladjem

Transcriptional silencing, one of the major impediments to gene therapy in humans, is often accompanied by replication during late S-phase. We report that transcriptional silencing and late replication were prevented by DNA sequences that can initiate DNA replication (replicators).