Nature Biotechnology 24, 555 - 557 (2006)
Published online: 16 April 2006; | doi:10.1038/nbt1201
A liposome-PCR assay for the ultrasensitive detection of biological toxinsJeffrey T Mason1, Lixin Xu1, 4, 5, Zong-mei Sheng2, 5
& Timothy J O'Leary31
Department of Biophysics, Armed Forces Institute of Pathology, 1413 Research Boulevard, Rockville, Maryland 20850, USA. 2
Department of Molecular Pathology, Armed Forces Institute of Pathology, 1413 Research Boulevard, Rockville, Maryland 20850, USA. 3
Biomedical Laboratory Research and Development Service, Veterans Health Administration, 810 Vermont Avenue, NW, Washington, DC 20420, USA. 4
Present address: Division of Monoclonal Antibodies, Center for Drug Evaluation and Research, US Food and Drug Administration, 29 Lincoln Drive, Bethesda, Maryland 20892, USA. 5
These authors contributed equally to this work.
Correspondence should be addressed to Jeffrey T Mason mason@afip.osd.mil We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent, the liposomes are ruptured to release the reporters, which are quantified by real-time PCR. Assays for cholera and botulinum toxins are several orders of magnitude more sensitive than current detection methods.
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