Nature Biotechnology 24, 461 - 465 (2006)
Published online: 19 March 2006; | doi:10.1038/nbt1191
Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue lightNadya G Gurskaya1, Vladislav V Verkhusha2, Alexander S Shcheglov1, Dmitry B Staroverov3, Tatyana V Chepurnykh3, Arkady F Fradkov1, Sergey Lukyanov1
& Konstantin A Lukyanov11
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. 2
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. 3
Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Correspondence should be addressed to Konstantin A Lukyanov kluk@ibch.ru Green fluorescent protein (GFP) and GFP-like proteins represent invaluable genetically encoded fluorescent probes1,
2. In the last few years a new class of photoactivatable fluorescent proteins (PAFPs) capable of pronounced light-induced spectral changes have been developed3. Except for tetrameric KFP1 (ref. 4), all known PAFPs, including PA-GFP5, Kaede6, EosFP7, PS-CFP8, Dronpa9, PA-mRFP110 and KikGR11 require light in the UV-violet spectral region for activation through one-photon excitation—such light can be phototoxic to some biological systems12. Here, we report a monomeric PAFP, Dendra, derived from octocoral Dendronephthya sp. and capable of 1,000- to 4,500-fold photoconversion from green to red fluorescent states in response to either visible blue or UV-violet light. Dendra represents the first PAFP, which is simultaneously monomeric, efficiently matures at 37 °C, demonstrates high photostability of the activated state, and can be photoactivated by a common, marginally phototoxic, 488-nm laser line. We demonstrate the suitability of Dendra for protein labeling and tracking to quantitatively study dynamics of fibrillarin and vimentin in mammalian cells.
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