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Nature Biotechnology 24, 351 - 357 (2006)
Published online: 26 February 2006; | doi:10.1038/nbt1197

CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells

Daniella Herszfeld, Ernst Wolvetang, Emma Langton-Bunker, Tung-Liang Chung, Adam A Filipczyk, Souheir Houssami, Pegah Jamshidi, Karen Koh, Andrew L Laslett, Anna Michalska, Linh Nguyen, Benjamin E Reubinoff, Irene Tellis, Jonathan M Auerbach, Carol J Ording, Leendert H J Looijenga & Martin F Pera

 
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A-Z product listing
647 goat anti-mouse IgG2b (Alexa Fluor)
647 goat anti-mouse IgM (Alexa Fluor)
Alexa Fluor 546 (Molecular Probes)
Alexa Fluor 568 (Invitrogen)
Alexa Fluor 647 (Molecular Probes)
C-terminal epitope in CD30 (Santa Cruz Biotechnology)
Cell Dissociation Solution (Sigma)
DC200 software (Leica)
digital camera (Olympus)
double-label stains, control mouse serum, monoclonal antibody GCTM-2, biotinylated rabbit anti-mouse IgM (DAKO)
ECL plus chemiluminescence system (Amersham Biosciences)
eight-well chamber slides (Nalgene-Nunc)
fluorescein isothiocyanate (Chemicon)
Fugene (Roche)
full-length human cDNA probe (Immunex Corporation)
goat anti-mouse IgG1 (Alexa Fluor)
green fluorescent chromosome 12 probe (Vysis)
Hybond-P (Amersham Biosciences)
luciferase construct (Stratagene)
M67 (Immunex Corporation)
Matrigel (BD-Biosciences)
monoclonal antibodies Ber-H2 (DAKO)
Texas Red–streptavidin (Amersham Biosciences)
TUNEL (Roche Diagnostics)
VECTASHIELD (Vector Laboratories)
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