Nature Biotechnology24, 358 - 362 (2006)
Published online: 12 February 2006; | doi:10.1038/nbt1187
An in vitro fluorescence screen to identify antivirals that disrupt hepatitis B virus capsid assembly
Stephen J Stray, Jennifer M Johnson, Benjamin G Kopek
& Adam Zlotnick
Figure 2.Fluorescence quenching correlates with size-exclusion chromatography at equilibrium. Assembly reactions containing 3 M C150BO and the indicated concentrations of NaCl were analyzed by fluorescence, 90° light scattering or size-exclusion chromatography at 24 h. Assembly reactions appeared to have equilibrated by this time. Note that fluorescence and light scattering data were normalized to their maximum values (at 0 NaCl for fluorescence, at 1 M NaCl for light scattering) for ease of comparison. Assembly data are expressed as total protein present in assembly products (capsid plus intermediate). Data are average standard deviation of three replicates, including samples from Figure 1. All fluorescence measurements were made in black 96-well fluorescence plates. LS, light scattering; F, fluorescence.
M C150BO and the indicated concentrations of NaCl were analyzed by fluorescence, 90° light scattering or size-exclusion chromatography at 24 h. Assembly reactions appeared to have equilibrated by this time. Note that fluorescence and light scattering data were normalized to their maximum values (at 0 NaCl for fluorescence, at 1 M NaCl for light scattering) for ease of comparison. Assembly data are expressed as total protein present in assembly products (capsid plus intermediate). Data are average 
standard deviation of three replicates, including samples from Figure 1. All fluorescence measurements were made in black 96-well fluorescence plates. LS, light scattering; F, fluorescence.
