Nature Biotechnology24, 358 - 362 (2006)
Published online: 12 February 2006; | doi:10.1038/nbt1187
An in vitro fluorescence screen to identify antivirals that disrupt hepatitis B virus capsid assembly
Stephen J Stray, Jennifer M Johnson, Benjamin G Kopek
& Adam Zlotnick
Figure 1.Strong correlation of real-time light scattering and fluorescence signals for assembly of BODIPY-FL–labeled C150. (a) Assembly of 3 M C150BO was measured by 90° light scattering at 21 °C. All measurements were carried out in a cuvette. Assembly was initiated manually at 90 s. Final NaCl concentrations (M) are indicated to the right of the panel. (b) Assembly monitored by fluorescence (excitation at 504 nm, emission at 509 nm). Final NaCl concentrations (M) are indicated to the right of the panel. (c) Alignment of light scattering and fluorescence signals from assembly reactions at 0.25 M NaCl (data from panels a and b). To allow direct comparison, we rescaled fluorescence data by applying the linear best fit of light scattering versus fluorescence curve (d) to the primary fluorescence data. Note that the scale for fluorescence has been inverted. (d) Comparison of fluorescence and light scattering from 100–500 s of assembly reactions using different NaCl concentrations. Final NaCl concentration (M) is indicated next to each data set. Note that the fluorescence scale has been inverted. Points lying off the diagonal correspond to peaks in light scattering probably caused by dust contaminants. LS, light scattering; F, fluorescence; c.p.s., counts per second.
M C150BO was measured by 90° light scattering at 21 °C. All measurements were carried out in a cuvette. Assembly was initiated manually at 90 s. Final NaCl concentrations (M) are indicated to the right of the panel. (b) Assembly monitored by fluorescence (excitation at 504 nm, emission at 509 nm). Final NaCl concentrations (M) are indicated to the right of the panel. (c) Alignment of light scattering and fluorescence signals from assembly reactions at 0.25 M NaCl (data from panels a and b). To allow direct comparison, we rescaled fluorescence data by applying the linear best fit of light scattering versus fluorescence curve (d) to the primary fluorescence data. Note that the scale for fluorescence has been inverted. (d) Comparison of fluorescence and light scattering from 100–500 s of assembly reactions using different NaCl concentrations. Final NaCl concentration (M) is indicated next to each data set. Note that the fluorescence scale has been inverted. Points lying off the diagonal correspond to peaks in light scattering probably caused by dust contaminants. LS, light scattering; F, fluorescence; c.p.s., counts per second.
