Nature Biotechnology 24, 185 - 187 (2006)
Published online: 1 January 2006; | doi:10.1038/nbt1177
In the supplementary information originally published online, Supplementary Table 1 contained an error. The table has been replaced and the corrected table appears below.Derivation of human embryonic stem cells in defined conditionsTenneille E Ludwig1, 2, 3, Mark E Levenstein1, Jeffrey M Jones1, 4, W Travis Berggren1, Erika R Mitchen1, Jennifer L Frane2, 3, Leann J Crandall1, Christine A Daigh2, 3, Kevin R Conard1, Marian S Piekarczyk1, Rachel A Llanas1
& James A Thomson1, 2, 3, 4, 51
WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA. 2
The Genome Center of Wisconsin, 425 Henry Mall, Madison, Wisconsin 53706, USA. 3
The Wisconsin National Primate Research Center, University of Wisconsin-Madison, 1220 Capitol Court, Madison, Wisconsin 53715-1299, USA. 4
Department of Obstetrics and Gynecology, University of Wisconsin-Madison Medical School, 600 Highland Avenue, Madison, Wisconsin 53792-6188, USA. 5
The Department of Anatomy, University of Wisconsin-Madison Medical School, 470 N. Charter Street, Madison, Wisconsin 53706-1509, USA.
Correspondence should be addressed to James A Thomson Thomson@primate.wisc.edu We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
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