Nature Biotechnology
- 24, 1569 - 1571 (2006)
Published online: 19 November 2006; | doi:10.1038/nbt1264
Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted libraryMarco A Mena1, Thomas P Treynor4, Stephen L Mayo2, 4 & Patrick S Daugherty1, 2, 31
Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA. 2
Institute for Collaborative Biotechnologies, University of California, Santa Barbara, California 93106, USA. 3
Biomolecular Science and Engineering, University of California, Santa Barbara, California 93106, USA. 4
Division of Biology, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Correspondence should be addressed to Patrick S Daugherty psd@engineering.ucsb.edu The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
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