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Nature Biotechnology - 24, 1429 - 1435 (2006)
Published online: 24 September 2006; | doi:10.1038/nbt1246

Compact, universal DNA microarrays to comprehensively determine transcription-factor binding site specificities

Michael F Berger, Anthony A Philippakis, Aaron M Qureshi, Fangxue S He, Preston W Estep III & Martha L Bulyk

Supplementary Fig. 1 (pdf 572K)
Survey of binding sites in the JASPAR database.

Supplementary Fig. 2 (pdf 516K)
Reproducibility of Cy3 dUTP signal intensities.

Supplementary Fig. 3 (pdf 680K)
Correlation of PBM signal intensities with affinities.

Supplementary Fig. 4 (pdf 632K)
Effects of binding site position and orientation on PBM signal.

Supplementary Fig. 5 (pdf 656K)
Comparison of median signal intensities for 28 Zif268 variants for fixed versus variable position, orientation, and flanking sequence.

Supplementary Fig. 6 (pdf 300K)
Correspondence between median signal intensities for 7-mers on distinct de Bruijn sequences.

Supplementary Fig. 7 (pdf 924K)
Biacore measurements supporting interdependence between the first two positions of the Cbf1 binding site.

Supplementary Fig. 8 (pdf 656K)
Minimum number of unique features on an array for different values of k.

Supplementary Fig. 9 (pdf 612K)
Dependence of Cy3 dUTP incorporation upon sequence context.

Supplementary Methods (doc 76K)


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Nature Biotechnology
ISSN: 1087-0156
EISSN: 1546-1696
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