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Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells

Nature Biotechnology volume 24, pages 1255–1256 (2006)Cite this article

Abstract

Erythroblast enucleation is thought to be largely dependent on signals mediated by other cells, such as macrophages. In an attempt to improve the in vitro production of red blood cells (RBCs) from immature hematopoietic progenitor cells, we have developed a method to produce enucleated RBCs efficiently in the absence of feeder cells. Our method may represent an efficient way to produce transfusable RBCs on a large scale from hematopoietic progenitors.

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Figure 1: Production of erythroid cells from CD34+ hematopoietic stem and progenitor cells isolated from human umbilical cord blood.

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Acknowledgements

We thank the Stem Cell Resource Network for providing cord blood.

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Authors and Affiliations

  1. Cell Engineering Division, RIKEN BioResource Center, Koyadai 3-1-1, Tsukuba, 305-0074, Ibaraki, Japan

    Kenichi Miharada, Takashi Hiroyama, Kazuhiro Sudo & Yukio Nakamura

  2. Division of Hematology, Institute of Clinical Medicine, University of Tsukuba, Ten-nodai 1-1-1, Tsukuba, 305-8577, Ibaraki, Japan

    Kenichi Miharada & Toshiro Nagasawa

Authors
  1. Kenichi Miharada
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  2. Takashi Hiroyama
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  3. Kazuhiro Sudo
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  4. Toshiro Nagasawa
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  5. Yukio Nakamura
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Contributions

K.M. designed and performed research and wrote the manuscript; T.H. and K.S. provided expertise; T.N. provided useful discussion; Y.N. coordinated research, analyzed data and wrote the manuscript.

Corresponding author

Correspondence to Yukio Nakamura.

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Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Flow cytometry analysis (CD34+ cells). (PDF 60 kb)

Supplementary Fig. 2

Effect of vascular endothelial growth factor (VEGF) and insulin-like growth factor-II (IGF-II) on cell expansion in passage I. (PDF 50 kb)

Supplementary Fig. 3

Mean cell amplification. (PDF 46 kb)

Supplementary Fig. 4

Flow cytometry analysis (CD71 and Gly-A). (PDF 110 kb)

Supplementary Fig. 5

Morphology of the cells produced following passage III. (PDF 1499 kb)

Supplementary Fig. 6

Effect of MAP (mannitol, adenine, and phosphate) in passage IV (enucleation step). (PDF 4379 kb)

Supplementary Fig. 7

Flow cytometry analysis (Rh-D). (PDF 56 kb)

Supplementary Methods (DOC 41 kb)

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Miharada, K., Hiroyama, T., Sudo, K. et al. Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells. Nat Biotechnol 24, 1255–1256 (2006). https://doi.org/10.1038/nbt1245

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  • DOI: https://doi.org/10.1038/nbt1245

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