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Nature Biotechnology 24, 1253–1254 (1 October 2006) | doi:10.1038/nbt1249

Protein chip fabrication by capture of nascent polypeptides

Sheng-Ce Tao & Heng Zhu

The most challenging step in protein microarray fabrication is high-throughput production of proteins. Here we report two similar strategies to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro–transcribed RNAs.