Nature Biotechnology 24, 76 - 77 (2005)
Published online: 4 December 2005; | doi:10.1038/nbt1170
Production of a recombinant bacterial lipoprotein in higher plant chloroplastsKarin Glenz1, Bernadette Bouchon2, Thomas Stehle3, Reinhard Wallich4, Markus M Simon3
& Heribert Warzecha11
Department of Pharmaceutical Biology, Julius-von-Sachs-Institut für Biowissenschaften, Julius-Maximilians Universität Wuerzburg, Julius-von-Sachs-Platz 2, 97082 Wuerzburg, Germany. 2
UMR 484-Inserm-Universite d'Auvergne, Rue Montalembert, 63005 Clermont-Ferrand, France. 3
Metschnikoff Laboratory, Max-Planck-Institute for Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany. 4
Institute for Immunology, University of Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany.
Correspondence should be addressed to Heribert Warzecha warzecha@biozentrum.uni-wuerzburg.de Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)—the basic constituent of the first generation monovalent human vaccine against Lyme disease. The recombinant OspA exhibits a lipid modification typical for bacteria and induced protective antibodies in mice, demonstrating that functionally active bacterial lipoproteins can be processed in plants.
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