Abstract
Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)—the basic constituent of the first generation monovalent human vaccine against Lyme disease. The recombinant OspA exhibits a lipid modification typical for bacteria and induced protective antibodies in mice, demonstrating that functionally active bacterial lipoproteins can be processed in plants.
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Acknowledgements
This work was funded by the Deutsche Forschungsgemeinschaft (WA 1448/2; SI 214/9-2).
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Supplementary information
Supplementary Fig. 1
Immunoblot analysis of the accumulation of rcOspA and rcOspA-T in tobacco leaves. (PDF 1099 kb)
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Glenz, K., Bouchon, B., Stehle, T. et al. Production of a recombinant bacterial lipoprotein in higher plant chloroplasts. Nat Biotechnol 24, 76–77 (2006). https://doi.org/10.1038/nbt1170
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DOI: https://doi.org/10.1038/nbt1170
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