 | Figure 1
Nature Biotechnology
23, 1126 - 1136 (2005)
Published online: 7 September 2005; | doi:10.1038/nbt1142
Engineered antibody fragments and the rise of single domainsPhilipp Holliger
& Peter J Hudson | | | | Figure 1. Schematic representation of different antibody formats, showing intact 'classic' IgG molecules alongside camelid VhH-Ig and shark Ig-NAR immunoglobulins.
Camelid VhH-Ig and shark Ig-NARs are unusual immunoglobulin -like structures comprising a homodimeric pair of two chains of V-like and C-like domains (neither has a light chain), in which the displayed V domains bind target independently. Shark Ig-NARs comprise a homodimer of one variable domain (V-NAR) and five C-like constant domains (C-NAR). A variety of antibody fragments are depicted, including Fab, scFv, single-domain VH, VhH and V-NAR and multimeric formats, such as minibodies, bis-scFv, diabodies, triabodies, tetrabodies and chemically conjugated Fab' multimers (sizes given in kilodaltons are approximate).
Katie Ris |
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