Nature Biotechnology
23, 873 - 878 (2005)
Published online: 26 June 2005; | doi:10.1038/nbt1110
In the print version of this article and the version originally published online, there is an error in Figure 3c. The figure has been replaced with a corrected figure in the PDF version of the article.Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5Ian T Paulsen1, Caroline M Press2, Jacques Ravel1, Donald Y Kobayashi3, Garry S A Myers1, Dmitri V Mavrodi4, Robert T DeBoy1, Rekha Seshadri1, Qinghu Ren1, Ramana Madupu1, Robert J Dodson1, A Scott Durkin1, Lauren M Brinkac1, Sean C Daugherty1, Stephen A Sullivan1, Mary J Rosovitz1, Michelle L Gwinn1, Liwei Zhou1, Davd J Schneider5, Samuel W Cartinhour5, William C Nelson1, Janice Weidman1, Kisha Watkins1, Kevin Tran1, Hoda Khouri1, Elizabeth A Pierson6, Leland S Pierson III6, Linda S Thomashow7
& Joyce E Loper21
The Institute for Genomic Research, Rockville, Maryland, USA. 2
US Department of Agriculture, Agricultural Research Service, Horticultural Crops Research Laboratory, Corvallis, Oregon, USA. 3
Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA. 4
Department of Plant Pathology, Washington State University, Pullman, Washington, USA. 5
US Department of Agriculture, Agricultural Research Service, Ithaca, New York, USA. 6
Department of Plant Sciences, University of Arizona, Tucson, Arizona, USA. 7
US Department of Agriculture, Agricultural Research Service, Root Disease and Biological Control Research Unit, Pullman, Washington, USA.
Correspondence should be addressed to Joyce E Loper loperj@mail.science.oregonstate.edu or Ian T Paulsen ipaulsen@tigr.org
Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
