Journal home
Advance online publication
Current issue
Archive
Press releases
Supplements
Focuses
Conferences
Guide to authors
Online submissionOnline submission
Permissions
For referees
Free online issue
Contact the journal
Subscribe
Advertising
work@npg
naturereprints
About this site
For librarians
 
NPG Resources
Bioentrepreneur
Nature Reviews Drug Discovery
Nature
Nature Medicine
Nature Genetics
Nature Reviews Genetics
Nature Methods
Nature Chemical Biology
news@nature.com
Clinical Pharmacology & Therapeutics
Nature Conferences
NPG Subject areas
Biotechnology
Cancer
Chemistry
Clinical Medicine
Dentistry
Development
Drug Discovery
Earth Sciences
Evolution & Ecology
Genetics
Immunology
Materials Science
Medical Research
Microbiology
Molecular Cell Biology
Neuroscience
Pharmacology
Physics
Browse all publications
Letters
Nature Biotechnology  23, 746 - 751 (2005)
Published online: 22 May 2005; | doi:10.1038/nbt1099

A high-throughput assay for assessing the cell permeability of combinatorial libraries

Peng Yu1, 2, Bo Liu1, 2 & Thomas Kodadek1

1  Departments of Internal Medicine and Molecular Biology and the Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390-8573, USA.

2  These authors contributed equally to this work.

Correspondence should be addressed to Thomas Kodadek thomas.kodadek@utsouthwestern.edu
There is great interest in the identification of synthetic molecules that are capable of manipulating protein-protein interactions in living cells. Peptides, unlike other classes of small molecules, have binding properties appropriate for this application, but most are poorly cell permeable and sensitive to proteases. Therefore, considerable effort has been expended in the development of libraries of oligomeric peptide-like molecules1, 2, 3, 4. However, there are no clear-cut rules to guide the design of libraries rich in cell permeable compounds. Furthermore, currently available empirical methods to assess permeability may not accurately reflect true permeability and/or are capable of only modest throughput5, 6, 7, 8, 9, 10, 11, 12. We describe here an assay for assessing the relative cell permeability of synthetic molecules in the context of steroid fusions that is capable of high throughput and can be used in any transfectable cell line.


MORE ARTICLES LIKE THIS

These links to content published by NPG are automatically generated.

 Top
natureproducts

Natureproducts is an online service detailing information about specific products used in this article, you can view the product descriptions, request information and compare with other similar products. The products used are listed in alphabetical order.

A-Z product listingbiocompare
Competitor Assay Kit (Invitrogen)
dual-luciferase reporter assay system (Promega)
polystyrene A-RAM macrobeads (Rapp Polymere)
See more natureproducts
 Top
 Top
Abstract
Previous | Next
Table of contents
Full textFull text
Download PDFDownload PDF
Send to a friendSend to a friend
Save this linkSave this link
Figures & Tables
Supplementary info
Products
Export citation
natureproducts

Search buyers guide:

 
ADVERTISEMENT
 
Nature Biotechnology
ISSN: 1087-0156
EISSN: 1546-1696
Journal home | Advance online publication | Current issue | Archive | Press releases | Supplements | Focuses | Conferences | For authors | Online submission | Permissions | For referees | Free online issue | About the journal | Contact the journal | Subscribe | Advertising | work@npg | naturereprints | About this site | For librarians
Nature Publishing Group, publisher of Nature, and other science journals and reference works©2005 Nature Publishing Group | Privacy policy