Nature Biotechnology
23, 457 - 462 (2005)
Published online: 20 March 2005; | doi:10.1038/nbt1081
Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNAAdam D Judge, Vandana Sood, Janet R Shaw, Dianne Fang, Kevin McClintock
& Ian MacLachlanSupplementary Fig. 1 (pdf 76K)
In vitro and In vivo properties of lipid encapsulated siRNA. Supplementary Fig. 2 (pdf 76K)
IFN- responses to encapsulated siRNA in different mouse strains and with alternate liposomal compositions. Supplementary Fig. 3 (pdf 92K)
Inflammatory cytokine production by monocytes in response to siRNA. Supplementary Fig. 4 (pdf 80K)
Immune stimulatory activity of siRNA is not due to contaminating ssRNA. Supplementary Fig. 5 (pdf 88K)
Sequence modifications to stimulatory siRNA can ameliorate systemic toxicities. Supplementary Fig. 6 (pdf 68K)
Stimulatory siRNA do not activate HEK293 Cells transfected with human TLR3, TLR7, TLR8 or TLR9. Supplementary Fig. 7 (pdf 72K)
PAGE purification of siRNA does not affect its immunostimulatory activity. Supplementary Table 1 (pdf 76K)
siRNA sequences used in these studies.
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