Nature Biotechnology23, 337 - 343 (2005)
Published online: 20 February 2005; | doi:10.1038/nbt1069
Programmable ligand-controlled riboregulators of eukaryotic gene expression
Travis S Bayer
& Christina D Smolke
Division of Chemistry and Chemical Engineering, 1200 East California Blvd., MC 210-41, California Institute of Technology, Pasadena, California 91125, USA.
Recent studies have demonstrated the importance of noncoding RNA elements in regulating gene expression networks1,
2. We describe the design of a class of small trans-acting RNAs that directly regulate gene expression in a ligand-dependent manner. These allosteric riboregulators, which we call antiswitches, are made fully tunable and modular by rational design. They offer flexible control strategies by adopting active or inactive forms in response to ligand binding, depending on their design. They can be tailor-made to regulate the expression of target transcripts in response to different cellular effectors. Coupled with in vitro selection technologies for generating nucleic acid ligand-binding species3,
4, antiswitches present a platform for programming cellular behavior and genetic networks with respect to cellular state and environmental stimuli.
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
Natureproducts is an online service detailing information about specific
products used in this article, you can view the product descriptions, request
information and compare with other similar products. The products
used are listed in alphabetical order.
