Nature Biotechnology
23, 227 - 231 (2004)
Published online: 26 December 2004; | doi:10.1038/nbt1052
Synthetic shRNAs as potent RNAi triggersDespina Siolas, Cara Lerner, Julja Burchard, Wei Ge, Peter S Linsley, Patrick J Paddison, Gregory J Hannon
& Michele A ClearySupplementary Fig. 1 (pdf 104K)
The set of shRNAs containing 19 or 29 bp. stems coding a luciferase sequence and either bearing or lacking a 2 nt. 3' overhang were incubated with bacterial RNase III to verify their double-stranded nature. Supplementary Fig. 2 (pdf 68K)
Northern blotting indicates that siRNAs and 19mer and 29mer shRNAs all give rise to RISC. Supplementary Fig. 3 (pdf 140K)
Structures of synthetic RNAs used for comparing siRNA and shRNA. Supplementary Table 1 (pdf 36K)
Sequences of the siRNAs used in this study.
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