Nature Biotechnology23, 227 - 231 (2004)
Published online: 26 December 2004; | doi:10.1038/nbt1052
Synthetic shRNAs as potent RNAi triggers
Despina Siolas, Cara Lerner, Julja Burchard, Wei Ge, Peter S Linsley, Patrick J Paddison, Gregory J Hannon
& Michele A Cleary
Supplementary Fig. 1 (pdf 104K) The set of shRNAs containing 19 or 29 bp. stems coding a luciferase sequence and either bearing or lacking a 2 nt. 3' overhang were incubated with bacterial RNase III to verify their double-stranded nature.
Supplementary Fig. 2 (pdf 68K) Northern blotting indicates that siRNAs and 19mer and 29mer shRNAs all give rise to RISC.
