Nature Biotechnology
23, 222 - 226 (2004)
Published online: 26 December 2004; | doi:10.1038/nbt1051
Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacyDong-Ho Kim, Mark A Behlke, Scott D Rose, Mi-Sook Chang, Sangdun Choi
& John J RossiSupplementary Fig. 1 (pdf 232K) Supplementary Fig. 2 (pdf 128K)
ESI mass spectra of the 27mer duplex EGFPS1 27+0 before (top) and after (bottom) incubation with Dicer are shown. Supplementary Fig. 3 (pdf 160K)
Sequence specificity of Dicer substrate 27mer dsRNAs. Supplementary Fig. 4 (pdf 256K)
SiRNAs and Dicer substrate dsRNAs do not induce interferons or activate PKR or generate specific "off target effects". Supplementary Table 1 (pdf 116K)
Summary of oligonucleotide reagents. Supplementary Table 2 (pdf 108K)
Molecular weights of possible 21mer duplexes derived from the 27mer duplex by Dicer processing.
| 
