Access
To read this article in full you may need to log in, make a payment or gain access through a site license (see right).
Research
Nature Biotechnology 23, 232–237 (1 February 2005) | doi:10.1038/nbt1061
Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay
&
Abstract
Standard microarrays measure mRNA abundance, not mRNA synthesis, and therefore cannot identify the mechanisms that regulate gene expression. We have developed a method to overcome this limitation by using the salvage enzyme uracil phosphoribosyltransferase (UPRT) from the protozoan Toxoplasma gondii.
To read this article in full you may need to log in, make a payment or gain access through a site license (see right).
