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Nature Biotechnology 23, 94–101 (1 January 2005) | doi:10.1038/nbt1046

Immunoaffinity profiling of tyrosine phosphorylation in cancer cells

John Rush , Albrecht Moritz , Kimberly A Lee , Ailan Guo , Valerie L Goss , Erik J Spek , Hui Zhang , Xiang-Ming Zha , Roberto D Polakiewicz & Michael J Comb

Tyrosine kinases play a prominent role in human cancer, yet the oncogenic signaling pathways driving cell proliferation and survival have been difficult to identify, in part because of the complexity of the pathways and in part because of low cellular levels of tyrosine phosphorylation. In general, global phosphoproteomic approaches reveal small numbers of peptides containing phosphotyrosine.