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Nature Biotechnology 22, 1146–1149 (1 September 2004) | doi:10.1038/nbt998

5|[prime]|-end SAGE for the analysis of transcriptional start sites

Shin-ichi Hashimoto , Yutaka Suzuki , Yasuhiro Kasai , Kei Morohoshi , Tomoyuki Yamada , Jun Sese , Shinichi Morishita , Sumio Sugano & Kouji Matsushima

Identification of the mRNA start site is essential in establishing the full-length cDNA sequence of a gene and analyzing its promoter region, which regulates gene expression. Here we describe the development of a 5|[prime]|-end serial analysis of gene expression (5|[prime]| SAGE) that can be used to globally identify transcriptional start sites and the frequency of individual mRNAs.