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Nature Biotechnology 22, 871–876 (1 July 2004) | doi:10.1038/nbt979

Temperature-sensitive control of protein activity by conditionally splicing inteins

Martin P Zeidler , Change Tan , Yohanns Bellaiche , Sara Cherry , Sabine H|[auml]|der , Urte Gayko & Norbert Perrimon

Conditional or temperature-sensitive (TS) alleles represent useful tools with which to investigate gene function. Indeed, much of our understanding of yeast has relied on temperature-sensitive mutations which, when available, also provide important insights into other model systems.