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Article
Nature Biotechnology  22, 739 - 745 (2004)
Published online: 16 May 2004; | doi:10.1038/nbt972

Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants

Baoxiu Qi1, 4, Tom Fraser1, Sam Mugford2, 5, Gary Dobson3, Olga Sayanova2, 6, Justine Butler1, Johnathan A Napier2, 6, A Keith Stobart1 & Colin M Lazarus1

1  School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, England, UK.

2  Long Ashton Research Station, Long Ashton, Bristol, BS41 9AF, England, UK.

3  Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.

4  Current address: Department of Biology and Biochemistry, University of Bath, 4 South, Claverton Down, Bath, BA2 7AY, England, UK.

5  Present address: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, England, UK.

6  Current address: Rothamsted Research, Harpenden, Herts AL5 2JQ, England, UK.

Correspondence should be addressed to Baoxiu Qi bssbq@bath.ac.uk
We report the production of two very long chain polyunsaturated fatty acids, arachidonic acid (AA) and eicosapentaenoic acid (EPA), in substantial quantities in a higher plant. This was achieved using genes encoding enzymes participating in the omega3/6 Delta8-desaturation biosynthetic pathways for the formation of C20 polyunsaturated fatty acids. Arabidopsis thaliana was transformed sequentially with genes encoding a Delta9-specific elongating activity from Isochrysis galbana, a Delta8-desaturase from Euglena gracilis and a Delta5-desaturase from Mortierella alpina. Instrumental in the successful reconstitution of these C20 polyunsaturated fatty acid biosynthetic pathways was the I. galbana C18-Delta9-elongating activity, which may bypass rate-limiting steps present in the conventional Delta6-desaturase/elongase pathways. The accumulation of EPA and AA in transgenic plants is a breakthrough in the search for alternative sustainable sources of fish oils.

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From alpha to omega?producing essential fatty acids in plants

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Nature Biotechnology
ISSN: 1087-0156
EISSN: 1546-1696
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