Research abstract

Article abstract


Nature Biotechnology 22, 695 - 700 (2004)
Published online: 2 May 2004 | doi:10.1038/nbt967



There is an Erratum (July 2004) associated with this Article.

There is a Corrigenda (July 2004) associated with this Article.

Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78

Diego Martinez1, Luis F Larrondo2, Nik Putnam1,3, Maarten D Sollewijn Gelpke1, Katherine Huang1, Jarrod Chapman1,3, Kevin G Helfenbein8, Preethi Ramaiya4, J Chris Detter1, Frank Larimer5, Pedro M Coutinho6, Bernard Henrissat6, Randy Berka4, Dan Cullen7 & Daniel Rokhsar1

This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike license (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation, and derivative works must be licensed under the same or similar license.


White rot fungi efficiently degrade lignin, a complex aromatic polymer in wood that is among the most abundant natural materials on earth. These fungi use extracellular oxidative enzymes that are also able to transform related aromatic compounds found in explosive contaminants, pesticides and toxic waste. We have sequenced the 30-million base-pair genome of Phanerochaete chrysosporium strain RP78 using a whole genome shotgun approach. The P. chrysosporium genome reveals an impressive array of genes encoding secreted oxidases, peroxidases and hydrolytic enzymes that cooperate in wood decay. Analysis of the genome data will enhance our understanding of lignocellulose degradation, a pivotal process in the global carbon cycle, and provide a framework for further development of bioprocesses for biomass utilization, organopollutant degradation and fiber bleaching. This genome provides a high quality draft sequence of a basidiomycete, a major fungal phylum that includes important plant and animal pathogens.

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  1. US DoE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 94598, USA.
  2. Departmento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontifica Universidad Católica de Chile and Millennium Institute for Fundamental and Applied Biology, Santiago, Chile.
  3. Department of Physics, University of California, Berkeley 94720, USA.
  4. Novozymes Biotech, 1445 Drew Avenue, Davis, California 95616, USA.
  5. Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
  6. Architecture et Fonction des Macromolécules Biologiques, UMR 6098, CNRS and Universités d'Aix-Marseille I & II, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
  7. USDA, Forest Service, Forest Products Laboratory, One Gifford Pinchot Dr., Madison, Wisconsin 53726, USA.
  8. Invertebrate Zoology, American Museum of Natural History, New York, New York 10027, USA.

Correspondence to: Dan Cullen7 e-mail: dcullen@facstaff.wisc.edu

Correspondence to: Daniel Rokhsar1 e-mail: DSRokhsar@lbl.gov