Nature Biotechnology
22, 560 - 567 (2004)
Published online: 11 April 2004; | doi:10.1038/nbt958
Adipose-derived adult stromal cells heal critical-size mouse calvarial defectsCatherine M Cowan1, Yun-Ying Shi1, Oliver O Aalami1, Yu-Fen Chou2, Carina Mari3, Romy Thomas1, Natalina Quarto1, Christopher H Contag4, Benjamin Wu2
& Michael T Longaker11
The Department of Surgery, Stanford University School of Medicine, Stanford University, 257 Campus Drive, Stanford, California
94305, USA. 2
The Department of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA. 3
The Department of Radiology, Division of Nuclear Medicine, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California
94305, USA. 4
The Department of Pediatrics, Stanford University School of Medicine, Stanford University, 318 Campus Drive, Stanford, California
94305, USA.
Correspondence should be addressed to Michael T Longaker longaker@Stanford.eduIn adults and children over two years of age, large cranial defects do not reossify successfully, posing a substantial biomedical burden. The osteogenic potential of bone marrow stromal (BMS) cells has been documented. This study investigates the in vivo osteogenic capability of adipose-derived adult stromal (ADAS) cells, BMS cells, calvarial-derived osteoblasts and dura mater cells to heal critical-size mouse calvarial defects. Implanted, apatite-coated, PLGA scaffolds seeded with ADAS or BMS cells produced significant intramembranous bone formation by 2 weeks and areas of complete bony bridging by 12 weeks as shown by X-ray analysis, histology and live micromolecular imaging. The contribution of implanted cells to new bone formation was 84−99% by chromosomal detection. These data show that ADAS cells heal critical-size skeletal defects without genetic manipulation or the addition of exogenous growth factors.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
