Nature Biotechnology
22, 547 - 553 (2004)
Published online: 4 April 2004; | doi:10.1038/nbt956
The genome sequence of the extreme thermophile Thermus thermophilusAnke Henne1, Holger Brüggemann1, 5, Carsten Raasch1, 5, Arnim Wiezer1, Thomas Hartsch1, 5, Heiko Liesegang1, Andre Johann1, 5, Tanja Lienard1, 3, Olivia Gohl1, Rosa Martinez-Arias1, 5, Carsten Jacobi1, 5, Vytaute Starkuviene1, 5, Silke Schlenczeck2, Silke Dencker1, Robert Huber4, Hans-Peter Klenk1, 5, Wilfried Kramer2, Rainer Merkl2, Gerhard Gottschalk1, 3
& Hans-Joachim Fritz1, 21
Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, University of Göttingen, Grisebachstr. 8, 37077 Göttingen, Germany. 2
Department of Molecular Genetics and Preparative Molecular Biology, Institute of Microbiology and Genetics, University of Göttingen, Grisebachstr. 8, 37077 Göttingen, Germany. 3
Department of General and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Grisebachstr. 8, 37077 Göttingen, Germany. 4
University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany. 5
Present addresses: Institut Pasteur, 25−28, Rue du Docteur Roux, 75724 Paris Cedex 15, France (H.B.),Variom Biotechnology AG, Robert-Koch-Platz 11, 10115 Berlin, Germany (C.R.), Genedata, Maulbeerstrasse 46, 4016 Basel, Switzerland (T.H.), Ministerium für Umwelt, Keplerstrasse 18, 66117 Saarbrücken, Germany (A.J.), GBF Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, 38124 Braunschweig, Germany (R.M.-A.), BASF Aktiengesellschaft, ZHV-A 030, 67056 Ludwigshafen, Germany (C.J.), EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany (V.S.), e.gene Biotechnologie GmbH, Pöckinger Fussweg 7a, 82340 Feldafing, Germany (H.-P.K.).
Correspondence should be addressed to Anke Henne ahenne@g2l.bio.uni-goettingen.de
Thermus thermophilus HB27 is an extremely thermophilic, halotolerant bacterium, which was originally isolated from a natural thermal environment in Japan. This organism has considerable biotechnological potential; many thermostable proteins isolated from members of the genus Thermus are indispensable in research and in industrial applications. We present here the complete genome sequence of T. thermophilus HB27, the first for the genus Thermus. The genome consists of a 1,894,877 base pair chromosome and a 232,605 base pair megaplasmid, designated pTT27. The 2,218 identified putative genes were compared to those of the closest relative sequenced so far, the mesophilic bacterium Deinococcus radiodurans. Both organisms share a similar set of proteins, although their genomes lack extensive synteny. Many new genes of potential interest for biotechnological applications were found in T. thermophilus HB27. Candidates include various proteases and key enzymes of other fundamental biological processes such as DNA replication, DNA repair and RNA maturation.
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