Nature Biotechnology22, 435 - 439 (2004)
Published online: 14 March 2004; | doi:10.1038/nbt951
A nanoliter-scale nucleic acid processor with parallel architecture
Jong Wook Hong1, 4, Vincent Studer1, 2, 4, Giao Hang1, W French Anderson1, 3
& Stephen R Quake1
1
Department of Applied Physics, California Institute of Technology, Pasadena, California 91125, USA.
2
CNRS/LPN Laboratoire de Photonique et de Nanostructures, route de Nozay, 91460 Marcoussis, France.
3
Gene Therapy Laboratories, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA.
4
These authors contributed equally to this study.
Correspondence should be addressed to Stephen R Quake quake@caltech.edu
The purification of nucleic acids from microbial and mammalian cells is a crucial step in many biological and medical applications1. We have developed microfluidic chips for automated nucleic acid purification from small numbers of bacterial or mammalian cells. All processes, such as cell isolation, cell lysis, DNA or mRNA purification, and recovery, were carried out on a single microfluidic chip in nanoliter volumes without any pre- or postsample treatment. Measurable amounts of mRNA were extracted in an automated fashion from as little as a single mammalian cell and recovered from the chip. These microfluidic chips are capable of processing different samples in parallel, thereby illustrating how highly parallel microfluidic architectures can be constructed to perform integrated batch-processing functionalities for biological and medical applications.
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