Nature Biotechnology
22, 306 - 312 (2004)
Published online: 8 February 2004; | doi:10.1038/nbt941
Probing the precision of the mitotic clock with a live-cell fluorescent biosensorJoshua T Jones, Jason W Myers, James E Ferrell Jr
& Tobias MeyerSupplementary Movie 1. (mov 205k)
Location of the biosensor during mitosis. Asynchronous RBL-FMB cells were imaged every 15 minutes with a CFP/YFP spinning disc confocal system. 3 cells that enter mitosis are identified by the white arrows. The scale bar in the lower left corner represents 20 µm and the time is in hours:minutes in the lower right corner. Supplementary Movie 2. (mov 1.8m)
Localization of the biosensor as monitored by total internal reflection fluorescence (TIRF) microscopy. RBL-FMB cells were imaged with a prism based CFP/YFP TIRF system every two minutes. The rectangular region highlights one cell undergoing mitosis with six cells in total going through mitosis during the course of the time lapse. The scale bar in the lower left corner represents 20 µm and the time is in hours:minutes in the lower right corner. Supplementary Movie 3. (mov 6.4m)
A 10X TIRF view of cycling RBL-FMB cells. RBL-FMB cells were imaged with a prism based CFP/YFP TIRF system. 70 cells undergo mitosis in the field of view during this 5 hour experiment. 75 frames of 149 are represented here. The scale bar in the lower left corner represents 50 µm and the time is represented in hours:minutes in the lower right corner. Supplementary Movie 4. (mov 1.3m)
40X TIRF and transmitted light time lapse of cycling mitotic cells. RBL-FMB cells were imaged with a prism based CFP/YFP TIRF system with near simultaneous transmitted light exposures. The scale bar in the lower left corner represents ten mm and the time is represented in hours:minutes in the lower right corner. Supplementary Table 1 (pdf 22k)
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