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Nature Biotechnology 22, 1573–1578 (1 December 2004) | doi:10.1038/nbt1040

Negative feedback inhibition of HIV-1 by TAT-inducible expression of siRNA

Hoshang J Unwalla , Ming-Jie Li , James D Kim , Hai Tang Li , Ali Ehsani , Jessica Alluin & John J Rossi

Here we demonstrate that an inducible anti-HIV short hairpin RNA (shRNA) expressed from a Pol II promoter inhibits HIV-1 gene expression in mammalian cells. Our strategy is based on a promoter system in which the HIV-1 LTR is fused to the Drosophila hsp70 minimal heat shock promoter.