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Nature Biotechnology 22, 1583–1587 (1 December 2004) | doi:10.1038/nbt1036

Baculovirus expression system for heterologous multiprotein complexes

Imre Berger , Daniel J Fitzgerald & Timothy J Richmond

The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes comprising many subunits.