Nature Biotechnology
22, 1275 - 1281 (2004)
Published online: 19 September 2004; | doi:10.1038/nbt1010
There is a Corrigenda (December 2004) associated with this Article.
The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducensSoon Ho Hong1, 6, Jin Sik Kim1, 6, Sang Yup Lee1, Yong Ho In2, 5, Sun Shim Choi2, Jeong-Keun Rih3, Chang Hoon Kim3, Haeyoung Jeong3, 4, Cheol Goo Hur3
& Jae Jong Kim41
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Department of BioSystems, BioProcess Engineering Research Center and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 373−1 Guseong-dong, Yuseong-gu, Daejeon 305−701, Republic of Korea. 2
Bioinfomatix, Inc., The fifth floor, Nam Chang Bldg., 748−162 Yeoksam-dong, Gangnam-gu, Seoul 135−925, Republic of Korea. 3
Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oun-dong, Yuseong-gu, Daejeon 305−333, Republic of Korea. 4
GenoTech Corp., 461−6 Jeonmin-dong, Yuseong-gu, Daejeon 305−390, Republic of Korea. 5
IDRTech Inc., 461−6 Jeonmin-dong, Yuseong-gu, Daejeon 305−390, Republic of Korea. 6
These authors contributed equally to this work.
Correspondence should be addressed to Sang Yup Lee leesy@kaist.ac.krThe rumen represents the first section of a ruminant animal's stomach, where feed is collected and mixed with microorganisms for initial digestion. The major gas produced in the rumen is CO2 (65.5 mol%), yet the metabolic characteristics of capnophilic (CO2-loving) microorganisms are not well understood. Here we report the 2,314,078 base pair genome sequence of Mannheimia succiniciproducens MBEL55E, a recently isolated capnophilic Gram-negative bacterium from bovine rumen, and analyze its genome contents and metabolic characteristics. The metabolism of M. succiniciproducens was found to be well adapted to the oxygen-free rumen by using fumarate as a major electron acceptor. Genome-scale metabolic flux analysis indicated that CO2 is important for the carboxylation of phosphoenolpyruvate to oxaloacetate, which is converted to succinic acid by the reductive tricarboxylic acid cycle and menaquinone systems. This characteristic metabolism allows highly efficient production of succinic acid, an important four-carbon industrial chemical.
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