 | Figure 1
Nature Biotechnology
22, 43 - 44 (2004)
doi:10.1038/nbt0104-43
Protein interaction maps on the flyPeter Uetz
& Michael J Pankratz | | | | Figure 1. The awesome power of comparative interactomics.
A comparison of interaction maps identifies evolutionarily conserved pathways in fly and yeast, such as those involving reptin and pontin that are homologs of DNA helicases. Reptin has just one high-confidence interactor5, pontin (indicated by thick lines in the figure), whereas pontin returned two high-confidence proteins, one of which is reptin. Previous data showed reptin-pontin interactions in yeast, flies and vertebrates, and their role in chromatin remodeling8, but those interactions and complexes involved different proteins in each model. In multicellular organisms, the pair is involved in wingless/ -catenin signaling. The other high-confidence partner of pontin, Or67c, is a membrane olfactory receptor. Although yeast does not have wingless/-catenin homologs, it does have a membrane receptor for mating factor alpha, and mutants in reptin or pontin homologs show an impaired response to alpha factor8. Despite different interaction patterns in each model, comparative interactomics suggests both additional components in the pathway as well as the hypothesis that the ancestral role of reptin and pontin was in response to pheromone or chemical signals. Maps are from refs. 3 and 5.
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