Nature Biotechnology21, 1069 - 1074 (2003)
Published online: 17 August 2003; | doi:10.1038/nbt862
A deoxyribozyme-based molecular automaton
Milan N Stojanovic1
& Darko Stefanovic2
1
Division of Clinical Pharmacology and Experimental
Therapeutics, Department of Medicine, Columbia University, Box
84, 650W 168th Street, New York, New York
10032, USA.
2
Department of Computer Science, Farris Engineering
Center #157, University of New Mexico, Albuquerque,
New Mexico 87131, USA.
Correspondence should be addressed to Milan N Stojanovic mns18@columbia.edu
We describe a molecular automaton, called MAYA, which encodes a
version of the game of tic-tac-toe and interactively competes against a human
opponent. The automaton is a Boolean network of deoxyribozymes that
incorporates 23 molecular-scale logic gates and one constitutively active
deoxyribozyme arrayed in nine wells (33) corresponding to the game
board. To make a move, MAYA carries out an analysis of the input
oligonucleotide keyed to a particular move by the human opponent and indicates
a move by fluorescence signaling in a response well. The cycle of human player
input and automaton response continues until there is a draw or a victory for
the automaton. The automaton cannot be defeated because it implements a perfect
strategy.
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3) corresponding to the game
board. To make a move, MAYA carries out an analysis of the input
oligonucleotide keyed to a particular move by the human opponent and indicates
a move by fluorescence signaling in a response well. The cycle of human player
input and automaton response continues until there is a draw or a victory for
the automaton. The automaton cannot be defeated because it implements a perfect
strategy.
