Nature Biotechnology 21, 526 - 531 (2003)
Published online: 14 April 2003 | doi:10.1038/nbt820

Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis

Haruo Ikeda1, Jun Ishikawa2, Akiharu Hanamoto3, Mayumi Shinose3, Hisashi Kikuchi4, Tadayoshi Shiba5, Yoshiyuki Sakaki6,7, Masahira Hattori1,7 & Satoshi Ōmura3,8

This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial-Share Alike license (, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation, and derivative works must be licensed under the same or similar license.

Species of the genus Streptomyces are of major pharmaceutical interest because they synthesize a variety of bioactive secondary metabolites. We have determined the complete nucleotide sequence of the linear chromosome of Streptomyces avermitilis. S. avermitilis produces avermectins, a group of antiparasitic agents used in human and veterinary medicine. The genome contains 9,025,608 bases (average GC content, 70.7%) and encodes at least 7,574 potential open reading frames (ORFs). Thirty-five percent of the ORFs (2,664) constitute 721 paralogous families. Thirty gene clusters related to secondary metabolite biosynthesis were identified, corresponding to 6.6% of the genome. Comparison with Streptomyces coelicolor A3(2) revealed that an internal 6.5-Mb region in the S. avermitilis genome was highly conserved with respect to gene order and content, and contained all known essential genes but showed perfectly asymmetric structure at the oriC center. In contrast, the terminal regions were not conserved and preferentially contained nonessential genes.

S. avermitilis1 is a Gram-positive bacterium in the genus Streptomyces (family Streptomycetaceae, class Actinobacteria). Streptomycetes are unique among soil bacteria because they form filamentous mycelia, aerial hyphae, and conidial spores during their life cycle2. Unlike other eubacterial genomes, the chromosomes of Streptomycetes form linear structures, and both ends, containing unique terminal-inverted repeats, bind terminal proteins3. Linear chromosomes, the predominant genetic elements in eukaryotes, have also been identified in Borrelia burgdorferi4 and Agrobacterium tumefaciens5, 6, but no terminal-inverted repeats or terminal proteins have been identified in these chromosomes. Because of the diversity of their secondary metabolite production pathways, Streptomycetes are of great interest for the commercial production of a variety of antibiotics that are used in human and veterinary medicine and agriculture, as well as of antiparasitic agents, herbicides, pharmacologically active metabolites, and several enzymes important in the food industry and other industries7. Six complete genomic sequences of the class Actinobacteria are now available: two Mycobacterium tuberculosis strains, H37Rv8 and CDC15519; Mycobacterium leprae TN10 and Corynebacterium diphtheriae (, which are mammalian pathogens with circular chromosomes; Corynebacterium glutamicum11; and S. coelicolor A3(2)12 (taxonomically belongs to Streptomyces violaceoruber). The availability of these genomes makes it possible to compare pathogen sequences and secondary metabolite producers within the Actinobacteria, which will provide valuable information for the application of these microbes in industrial fields including drug discovery. Here we present the complete sequence of the S. avermitilis genome and a comparative analysis that explores the genetic features of the Streptomycetes.



Sequencing and gene annotation of the S. avermitilis genome.

The genome sequence of S. avermitilis was obtained by whole genome shotgun sequencing13 in combination with sequencing of additional cosmid clones. The principal features of the S. avermitilis chromosome and the linear plasmid SAP1 are summarized in Table 1. The linear chromosome contains 7,574 open reading frames (ORFs); we assigned a putative function to the encoded proteins for 4,563 (60.2%) of these. Of the remaining 3,011 ORFs, 2,738 (36.1%) showed similarity to ORFs encoding hypothetical proteins of unknown function annotated in other genomes, and 273 (3.6%) had no substantial similarity to data in the public databases. In contrast, when the S. coelicolor A3(2) genome was excluded from the analysis, 2,291 S. avermitilis ORFs (30.2%) had no substantial similarity to known genes. The plasmid SAP1 contains 96 ORFs, of which 33 (34.3%) encoded proteins that were assigned putative functions and 45 (46.9%) encoded proteins similar to proteins of unknown function annotated in other genomes. The remaining 18 ORFs (18.8%) had no substantial similarity to data in the public databases. The average GC content of the S. avermitilis chromosome was 70.7%, but some larger regions showed a consistently lower GC content (Fig. 1). For example, the six rRNA operons (16S-23S-5S rRNA) had GC content ranging from 57.75 to 58.01%. The rRNA operons are known to have similar GC content in all organisms, irrespective of average GC content, because of constraints on the composition of these functional RNA molecules. Transposons, including truncated forms, phage, and plasmid sequences, comprise 1.5% (99 putative transposase and 16 putative phage-plasmid integrase genes) of the S. avermitilis chromosome. Most of the mobile sequences (80 transposase and 7 integrase genes) were located in the regions near the two chromosomal ends, called subtelomeric regions (see below and Fig. 1).

Figure 1: Schematic representation of the S. avermitilis chromosome (A) and the linear plasmid SAP1 (B).

Figure 1 : Schematic representation of the S. avermitilis chromosome
			 (A) and the linear plasmid SAP1 (B).

Each replicon is drawn to scale in megabases (A) and kilobases (B). Lines indicate AseI restriction sites (i), distribution of genes according to direction of transcription (ii; + strand (upper), − strand (lower)), distribution of secondary metabolite gene clusters (iii), distribution of tRNAs (iv), distribution of rRNA operons (v), distribution of IS and transposase genes (vi), distribution of phage- or integral plasmid-related sequences (vii), GC % variation along the replicons (viii; nonoverlapping 5 kb window (A) and 1 kb window (B)), and GC skew (ix; 50 kb window and 10 kb step (A) and 5 kb window and 500 bp step (B)). The nucleotide sequence data reported in this paper appears in the DDBJ/EMBL/GenBank (NCBI) nucleotide sequence databases with the accession nos. AP005021AP005050, BA000030, and AP005645.

Full size image (55 KB)

Clustering of the 7,574 ORFs by the Basic Local Alignment Search Tool protein clustering program (BLASTCLUST; minimum 30% identity, minimum 80% length coverage) showed that 35% (2,664) clustered into 721 paralogous families, with membership ranging from 2 to 91 genes per family. Two large gene families were represented, one related to membrane-spanning components of the ATP binding cassette (ABC) transporters and the other related to two-component transcriptional regulator systems. We noted that S. avermitilis contains two rpoA genes (SAV440 and SAV4953) encoding the RNA polymerase alpha subunit. These two putative proteins are 96% identical (E value = 0) and show substantial homology with the RNA polymerase alpha subunit of S. coelicolor A3(2) (96% (E value = 0) and 100% (E value = 0), respectively), Streptomyces granaticolor (96% (E value = 0) and 99% (E value = 0), respectively), M. tuberculosis (78% (E value = 10−142) and 75% (E value = 10−144), respectively) and M. leprae (77% (E value = 10−140) and 74% (E value = 10−141), respectively). This suggested that both gene products may act as RNA polymerase alpha subunits in S. avermitilis. Fully sequenced eubacteria usually contain only one rpoA gene, and this may thus be the first case of two RNA polymerase alpha subunits being present in one bacterial genome. On the other hand, only one ORF each for rpoB, rpoC, and rpoZ was found in the S. avermitilis genome. We also identified 60 putative RNA polymerase sigma factors in the S. avermitilis chromosome, 47 of them belonging to the extracytoplasmic function (ECF) subfamily. S. coelicolor A3(2) also encodes 65 RNA polymerase sigma factors, of which 45 are ECF sigma factors12. The presence of numerous sigma factor genes may be a characteristic of the genus Streptomyces, as the next highest number known is 23 in Mesorhizobium loti14. The analysis of two RNA polymerase alpha subunits and numerous sigma factors is of importance for studying functional RNA polymerase complexes. All the annotated genes identified in this study are available on the authors' website (see URL in the Experimental Protocol).

Analysis of the linear genome structure.

We identified a putative origin of replication (oriC) at position 5,287,935–5,289,024 of the chromosome. This region contained at least 19 dnaA box-like sequences15, and the order of genes flanking the region is almost the same as that observed in circular bacterial chromosomes. The oriC of the linear chromosomes of B. burgdorferi4, A. tumefaciens5, 6, and S. coelicolor A3(2)12 is located in the middle of the chromosomes. In contrast, oriC on the S. avermitilis chromosome is shifted 776 kb away from the center and toward the right end. Although a GC-skew inversion is generally observed at the oriC of most bacterial chromosomes, no obvious GC-skew inversion could be detected on the S. avermitilis chromosome at any window size tested (Fig. 1A). Similarly, no GC-skew inversion has been observed in several other genomes, including Deinococcus radiodurans R116 and Haemophilus influenzae KW2013. The linear plasmid SAP1 showed no clear GC-skew inversion either but showed a marked bias in transcriptional direction of genes near the middle of the plasmid (Fig. 1B). In a few prokaryotes, including B. burgdorferi and A. tumefaciens, the termini of the linear replicons seem to be covalently closed by hairpin loop structures4, 5, 6. On the other hand, the termini of the Streptomyces chromosomes covalently bind proteins at the 5′ end3. Alignment of the terminal sequences of the S. avermitilis chromosome and SAP1 with other Streptomyces chromosome termini17 indicated extensive homology in the first 96 nucleotides (see Supplementary Fig. 1 online). Recently, genes encoding the terminal proteins of the linear plasmids of Streptomyces rochei have been cloned and shown to be similar to genes of putative terminal proteins of other Streptomyces18. Homology searching using these sequences for the S. avermitilis genome identified two putative terminal protein genes, tpgA1 and tpgA2, near the right end of the chromosome and the left end of SAP1, respectively. TpgA1 and TpgA2 showed 90% identity (E value = 3 × 10−92) to each other and substantial similarity to terminal proteins of other Streptomyces (60–85% identity) (see Supplementary Fig. 2 online).

Comparative analysis of S. avermitilis with S. coelicolor A3(2) and other bacteria.

We compared the theoretical proteome of S. avermitilis with those of other bacteria by using pairwise BLASTP searches without low-complexity filtering and defining reciprocal best-hit pairs as orthologs. We performed the comparative analysis with the publicly available protein sequences of S. coelicolor A3(2)12, M. tuberculosis (AL123456), Escherichia coli (U00096), and Bacillus subtilis (AL009126). We found that 5,283 (69%) genes have orthologs in S. coelicolor A3(2). A smaller number, 1,966 (26%), had orthologs in M. tuberculosis, and only 21% had orthologs in E. coli (1,593 genes) or B. subtilis (1,586 genes). A comparison of ORFs in S. avermitilis and S. coelicolor A3(2) showed that 2,291 (738 encoding for proteins with assigned function) and 2,307 (1,080 encoding for proteins with assigned function), respectively, did not show any substantial similarity and were thus unique to each species. These unique ORFs included genes encoding for proteins involved in secondary metabolism, degradation of polymers and xenobiotics, transcriptional regulation, and transposition (Table 2).


Some differences between the phenotypes of S. avermitilis and S. coelicolor A3(2) can be explained by the presence or absence of specific orthologs in either genome. For instance, S. avermitilis can grow in medium containing sucrose as a sole carbon source but S. coelicolor A3(2) cannot. As shown in Table 2, S. avermitilis contains a gene for a sucrose phosphotransferase enzyme (SAV3925) but S. coelicolor A3(2) lacks the ortholog. The presence of genes encoding enzymes involved in agar metabolism in S. coelicolor A3(2) (SCO3471, 5848, 5849) but not in S. avermitilis may explain the ability of the former to degrade agar and the lack of this capability in the latter. Both S. avermitilis and S. coelicolor A3(2)19 lack the recBC genes and their corresponding suppressors sbcAB. These genes are involved in recombination in E. coli. Alternative recD genes, SAV5329 and SCO2737, were found in both strains, which suggests that the traditional RecBCD pathway of homologous recombination is absent in Streptomyces but an alternative type of recombination pathway may be present. The S. avermitilis chromosome contains two putative genes (SAV2423 and SAV2442) encoding the topoisomerase IV subunits involved in the separation of circular daughter chromosomes. The corresponding genes in S. coelicolor A3(2) are SCO5822 and SCO5836. Both chromosomes lack a XerCD-like site-specific recombination system for resolving dimeric circular chromosomes20, a system commonly found in organisms with circular chromosomes. S. avermitilis lacks the operon for nitrate reductase (narG–J) whereas S. coelicolor A3(2) contains three copies of the nitrate reductase operon. The hydrogenase operons hypA–F and hydAB, which are known to be involved in nickel metabolism21 and in urease activity22 but have not been functionally characterized in detail, were found in S. avermitilis but not in S. coelicolor A3(2). Both S. avermitilis and S. coelicolor A3(2) have two genes—SAV3417/SAV4725 and SCO4839/SCO3334, respectively—encoding a tryptophanyl tRNA synthethase. SAV3417 showed significant similarity with the tryptophanyl tRNA synthetases of other microorganisms but only 48% identity (E value = 7 × 10−82) with that of SAV4725. Some data suggest that SAV4725 may be involved in a mechanism that renders some Streptomyces strains, including S. avermitilis and S. coelicolor A3(2), naturally resistant to indolmycin, an antibiotic that inhibits tryptophanyl tRNA synthase (ref. 23 and data not shown). S. avermitilis and S. coelicolor A3(2) are naturally resistant to chloramphenicol. S. coelicolor encodes antibiotic-transmembrane efflux proteins CmlR and CmlR2 (SCO7526 and SCO7662), which are also found in other Actinobacteria such as Streptomyces and Rhodococcus. S. avermitilis lacks orthologous genes for these efflux proteins but has a gene encoding the antibiotic-modifying enzyme chloramphenicol phosphotransferase (SAV877), which has been characterized in the chloramphenicol-producing Streptomyces venezuelae (Q56148). These data suggest that these organisms have different mechanisms for chloramphenicol resistance.

The oxidation reaction carried out by cytochrome P450 using molecular oxygen often functions in the detoxification and modification of low-molecular-weight compounds. Genes encoding cytochrome P450 are not abundant in most bacteria; usually 0–4 genes are found. S. avermitilis contains 33 putative cytochrome P450 genes. One of them, SAV575, and four ferredoxin genes are unique to S. avermitilis (Table 2). S. coelicolor A3(2) contains 18 putative cytochrome P450 genes24. In S. avermitilis, one-third of all cytochrome P450 genes (11 genes) may be involved in the biosynthesis of secondary metabolites. The remaining 22 ORFs of S. avermitilis and the 18 putative cytochrome P450 genes of S. coelicolor A3(2) may be involved in defense mechanisms against toxic compounds in the soil environment. With 1,553 of the S. avermitilis and 1,227 of the S. coelicolor A3(2) predicted hypothetical proteins not yet characterized, much remains to be elucidated about the genetic differences between these Streptomyces species.

We have previously reported that S. avermitilis possesses at least 25 kinds of secondary metabolite gene clusters25. Our analysis here identified five additional secondary metabolite gene clusters in the S. avermitilis chromosome—four involved in the biosynthesis of terpene compounds and one in the biosynthesis of polyketide compounds. We recently confirmed experimentally the production of some metabolites predicted in this and previous studies, including geosmin, pentalenolactone, squalene, and pentaene25 (data not shown). No secondary metabolite gene clusters were found on the plasmid SAP1. The total length of these 30 secondary metabolite gene clusters, containing 271 genes, was estimated to be 594 kb, indicating that 6.6% of the S. avermitilis genome is composed of genes encoding proteins involved in the biosynthesis of secondary metabolites (Fig. 1 and Supplementary Table 1 online). Many genes related to biosynthesis of secondary metabolites, including antibiotics, also seem to be less conserved among Streptomyces species and unique to S. avermitilis.

Overall genome structure of S. avermitilis.

S. avermitilis and S. coelicolor A3(2) showed conservation of linearity and gene order along their respective chromosomes (Fig. 2). However, most of the highly conserved internal regions show a structural asymmetry between the S. coelicolor A3(2) and S. avermitilis chromosomes when the oriCs are placed in the same direction at the center of each chromosome. Three regions, indicated by A, B and C in Fig. 2, were also of interest. All known essential genes are located in the 6.5-Mb highly conserved internal region (SAV1625–7142 in S. avermitilis and SCO1196–6804 in S. coelicolor A3(2), respectively). Gene content and location in the 6.5-Mb conserved region also showed syntenic features with the circular chromosomes of Actinobacteria, M. tuberculosis, C. diphtheriae, and C. glutamicum, as shown by comparison of the S. coelicolor A3(2) genome with the first two of these bacteria12. Marked synteny was also observed in the 5.2-Mb region from SAV2398–6905 in S. avermitilis and the 4.9-Mb region from SCO1440–5869 in S. coelicolor A3(2). Region B (position 4,313,571–4,51,925; SAV3480–3709) to the left of oriC in the S. avermitilis chromosome and the corresponding region (position 5,311,553–5,063,401; SCO3062–3251) to the right of oriC in the S. coelicolor A3(2) chromosome contains essential genes including those encoding enolase, phosphoenolpyruvate carboxylase, the galactose operon (galKET), and a 50S ribosomal protein. Region C (position 5,834,450–6,077,662; SAV4793–5019) to the right of oriC in the S. avermitilis chromosome and the corresponding position to the left of oriC in the S. coelicolor A3(2) chromosome (position 3,781,004–3,462,666; SCO4467–4784) also contains essential genes, including those encoding two NADH dehydrogenase nuo operons, rpoABC, several 30S and 50S ribosomal proteins, and groES–EL.

Figure 2: Synteny between S. avermitilis and S. coelicolor A3(2) linear chromosomes.

Figure 2 : Synteny between S. avermitilis and S. coelicolor
			 A3(2) linear chromosomes.

Each point in this figure is a reciprocal best hit. These hits were obtained by pairwise BLASTP searches of predicted S. avermitilis proteins against those of S. coelicolor A3(2) with a maximum expectation value of 10−20. Each protein pair is graphed according to the location of the corresponding gene on respective DNA molecules. The bars above and at right of plot indicate the region conserved to circular Actinobacterium chromosomes (green), subtelomeric (blue), and backbone (red) regions in S. avermitilis and S. coelicolor A3(2), respectively. Arrows indicate the position of oriC. A, B, and C indicate inverted regions between S. avermitilis and S. coelicolor A3(2).

Full size image (33 KB)

The analysis also revealed that the regions near both telomeres are less conserved both in sequence and in regard to ortholog distribution. These variable subtelomeric regions are located 2.0 Mb from the left telomere and 0.5 Mb from the right telomere in S. avermitilis. Corresponding subtelomeric regions can be found 1.1 Mb from both the left and right telomeres in S. coelicolor A3(2). The left 2-Mb subtelomeric region contained the partially conserved and inverted A region of the S. avermitilis chromosome (Fig. 2) (position 1,750,041–1,957,682; SAV1418–1593) corresponding to the right subtelomeric region of the S. coelicolor A3(2) chromosome (position 7,602,649–7,438,541; SCO1010–1168). This inverted region contains nonessential genes such as those encoding tagatose-bisphosphate aldolase, secreted α-galactosidase, NADPH-ferredoxin reductase, and uracil DNA glycosylase. The subtelomeric regions contained 1,020 of S. avermitilis-specific genes corresponding to 44.5% of all 2,291 specific genes in S. avermitilis. Similarly, 972 (42.1%) of the 2,307 S. coelicolor A3(2)–specific genes were located in the subtelomeric regions.

Out of the 30 gene clusters related to secondary metabolism found in S. avermitilis, 17 (57%) are located in the subtelomeric regions (Fig. 1A and Supplementary Table 1 online). S. avermitilis specific gene clusters for the avermectin (ave; position 1,132,045–1,212,960) and pentaene (pte; position 486,648–567,017) biosynthesis pathways are also located in the left 2-Mb subtelomeric region. In contrast, many gene clusters for secondary metabolites commonly produced by several Streptomyces species, including S. avermitilis, were located in the 6.5-Mb internal conserved region of the chromosome. For example, the three biosynthetic gene clusters for geosmin (geo; position 2,635,583–2,640,003), pentalenolactone (ptl; position 3,749,307–3,758,093), and oligomycin (olm; position 3,536,766–3,634,592) were located in the 6.5-Mb internal region.



We determined and analyzed the sequences of the 9.02-Mb chromosome and the plasmid SAP1 of S. avermitilis. The analysis of genomic sequence revealed at least 7,574 ORFs in the chromosome, of which 2,664 (35%) were shown to form 721 paralogous families, ranging from 2 to 91 genes per family. These results suggest that at least one-third of all S. avermitilis genes might have emerged by gene duplication during evolution. Two gene families in particular, the membrane-spanning components of the ABC transport systems and the family of two-component system transcriptional regulators, show this pattern. The paralogous families also included various genes involved in transcription, such as the RNA polymerase alpha subunit and numerous sigma factors, and genes encoding cytochrome P450, some of which may be involved in defense mechanisms against toxic compounds in the soil environment. The gene content of S. avermitilis suggests that its genome might have evolved by acquisition of novel gene functions to adapt to the extremely variable soil environment, the intense competition, and the drastic changes in physical conditions and nutrient availability. The abundance of such paralogous families was also observed in S. coelicolor A3(2) and may be characteristic of prototrophic bacteria in the genus Streptomyces.

Comparative analysis of S. avermitilis and S. coelicolor A3(2) revealed a 6.5-Mb, highly conserved internal region where most essential genes are located, with similar order and direction in the two species. This region also shows structural similarity to other circular bacterial chromosomes. This finding implies that the 6.5-Mb internal region is the underlying backbone of the Streptomyces chromosomes and may have evolved from an ancestor common to all bacteria with circular chromosomes. On the other hand, we also found variable and less conserved subtelomeric regions near both telomeres. Notably, more than half of the genes related to secondary metabolism were found in the subtelomeric regions, where no known essential gene was found. In addition, the subtelomeric regions contained most of the mobile elements in the genome and about half of the non-secondary-metabolism genes specific to S. avermitilis. A similar subtelomeric region organization was also found in S. coelicolor A3(2). These findings suggest that frequent gene duplication may have preferentially occurred in the subtelomeric regions of the Streptomyces chromosomes. This could also be why genes for common secondary metabolites are preferentially located in the internal region of the chromosome, whereas specific or unique genes such as ave are found in the subtelomeric region. The emergence of new genes and structural variability at particular loci, such as the subtelomeric regions, may be unique to linear bacterial chromosomes. The information and materials presented in this study will be of great use in improving and modifying Streptomyces for the production of secondary metabolites, including antibiotics.


Experimental protocol

Sequencing and assembly.

Whole genome shotgun cloning was done using ~2 kb inserts. Shotgun template DNA was prepared by direct PCR amplification of the insert from colonies as previously reported26 but with the following modifications to optimize amplification of the extremely high-GC DNA: dimethyl sulfoxide was added to final concentration of 5% and the denaturation temperature was 98 °C in Ex-Taq PCR cocktail (Takara Bio Inc., Shiga, Japan). Electrophoresis was done at reduced voltage, as this also gave better results than the standard protocol recommended by the manufacturer. Because of the high-GC homologous sequences, the first-round assembly of shotgun data using phred/phrap assembler ( resulted in numerous gaps and in low confidence levels for the assembled contigs. To overcome these problems, we constructed a cosmid library with ~40-kb DNA inserts. Cosmid end-sequences were used to estimate adjacent contig pairs and to evaluate correct assembly throughout the project. Cosmids were also used for gap filling by shotgun sequencing. In addition, PCR products were sequenced by primer walk to fill gaps and to resolve ambiguous regions. We also constructed AseI and DraI restriction maps of the S. avermitilis genome to confirm the final assembled data. In total, we assembled 186,619 random shotgun sequences from the whole genome, 10,787 cosmid-end sequences, 107 contig sequences from cosmid shotgun sequencing, 162 PCR-product sequences, and 72 manually curated sequences, achieving 13.3-fold coverage. The assembled data were consistent with positions and orientations of cosmid-end sequences. Both restriction sites in the assembled data were also in good agreement with the experimental data. Finally, the entire sequence was estimated to have an error rate of less than 1 per 10,000 bases (phrap score ≥ 40).

Gene finding and annotation.

ORFs were predicted with Glimmer27, 28. The program was trained with 2,000 ORFs larger than 500 bp from the genomic sequence of S. avermitilis as well as with the S. coelicolor A3(2) genes available from the public databases. We also used FramPlot29, BLAST30 (National Center for Biotechnology Information BLAST package;, and HmmPfam31 to confirm protein-coding genes predicted by Glimmer and found additional gene candidates.


All the annotated genes identified in this study are also available on the authors' website at

Note: Supplementary information is available on the Nature Biotechnology website.



We thank H. Shimizu, H. Horikawa, H. Nakazawa, and T. Osonoe for technical assistance. We also thank T.D. Taylor for critical reading of this paper. This work was supported in part by Grant-in-Aid of the Ministry of Education, Culture, Sports, Science and Technology, Japan, No. 13680676, Grant of Araki Memorial Foundation for Medical and Biochemical Studies, Grant of the 21st Century COE Program, Ministry of Education, Culture, Sports, Science and Technology, Japan, and the Research for the Future Program of the Japan Society for the Promotion of Science, No. 00L01411.

Competing interests statement

The authors declare no competing financial interests.

Received 22 October 2002; Accepted 22 January 2003; Published online 14 April 2003.



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  1. Kitasato Institute for Life Sciences, Kitasato University, Kanagawa 228-8555, Japan.
  2. National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
  3. The Kitasato Institute, Tokyo 108-8642, Japan.
  4. National Institute of Technology and Evaluation, Tokyo 151-0066, Japan.
  5. School of Science, Kitasato University, Kanagawa 228-8555, Japan.
  6. Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
  7. RIKEN Yokohama Institute, Genomic Sciences Center, Kanagawa 230-0045, Japan.
  8. Kitasato Institute for Life Sciences, Kitasato University, Tokyo 108-8641, Japan.

Correspondence to: Haruo Ikeda1 e-mail:

Correspondence to: Masahira Hattori1,7 e-mail: