Nature Biotechnology
21, 269 - 274 (2003)
Published online: 18 February 2003; | doi:10.1038/nbt794
Scanning the human genome with combinatorial transcription factor
librariesPilar Blancafort, Laurent Magnenat
& Carlos F. Barbas IIISupplementary Figure 1. (pdf 411K)
Analysis of A431 cells infected
with some of the selected pMX-TFsZF pools by flow cytometry from the
3ZF selections (A) or 6ZF selections (B). Blue represents A431
cells infected with the selected pMX-TFsZF pools and stained with the
corresponding antibody. Orange represents A431 cells infected with the 3ZF or
6ZF unselected libraries. Green represents mockinfected cells. The stippled
line indicates control staining without primary antibody. Supplementary Figure 2. (pdf 1.7M)
Specificity of isolated 3ZF
TFsZF clones VE-5, VE-8, VE-13 and VE-18 (A-D) and 6ZF
TFsZF 144-4, 144-5 and 144-13 (E-G) activating VE-cadherin
determined by FACS using a panel of 10 cell surface markers. Legend is as
described in figure 1. Supplementary Figure 3. (pdf 71K)
Luciferase transactivation assay of
all selected TFsZF with the VE-cadherin promoter. The amount of
effector TFsZF construct used in the assay is indicated. Supplementary Table 1 (pdf 14K) Supplementary Table 2 (pdf 17K) Supplementary Experimental Protocol (pdf 26K)
| 
