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Journal home Advance online publication Current issue Archive Press releases Supplements Focuses Conferences Guide to authors Online submission Permissions For referees Free online issue Contact the journal Subscribe Advertising work@npg naturereprints About this site For librarians   NPG Resources Bioentrepreneur Nature Reviews Drug Discovery Nature Nature Medicine Nature Genetics Nature Reviews Genetics Nature Methods Nature Chemical Biology news@nature.com Clinical Pharmacology & Therapeutics Nature Conferences NPG Subject areas Biotechnology Cancer Chemistry Clinical Medicine Dentistry Development Drug Discovery Earth Sciences Evolution & Ecology Genetics Immunology Materials Science Medical Research Microbiology Molecular Cell Biology Neuroscience Pharmacology Physics Browse all publications Article Nature Biotechnology  21, 47 - 51 (2002) Published online: 2 December 2002; | doi:10.1038/nbt767 Long-term multiple color imaging of live cells using quantum dot bioconjugates Jyoti K. Jaiswal1, Hedi Mattoussi2, J. Matthew Mauro3 & Sanford M. Simon1 1  The Rockefeller University, Box 304, 1230 York Avenue, New York, NY 10021, USA. 2  Division of Optical Sciences, U.S. Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA. 3  Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC 20375, USA. Correspondence should be addressed to Sanford M. Simon simon@rockefeller.eduLuminescent quantum dots (QDs)—semiconductor nanocrystals—are a promising alternative to organic dyes for fluorescence-based applications. We have developed procedures for using QDs to label live cells and have demonstrated their use for long-term multicolor imaging of live cells. The two approaches presented are (i) endocytic uptake of QDs and (ii) selective labeling of cell surface proteins with QDs conjugated to antibodies. Live cells labeled using these approaches were used for long-term multicolor imaging. The cells remained stably labeled for over a week as they grew and developed. These approaches should permit the simultaneous study of multiple cells over long periods of time as they proceed through growth and development. MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated NEWS AND VIEWS Enlightened receptor dynamics Nature Biotechnology News and Views (01 Feb 2004) RESEARCH Quantum dot ligands provide new insights into erbB/HER receptor–mediated signal transduction Nature Biotechnology Research (01 Feb 2004) Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots Nature Biotechnology Research (01 Jan 2003)  Top Abstract Previous | Next Table of contents Full text Download PDF Send to a friend Save this link Open Innovation Challenges Optimizing Sub-cellular Localization Tags Deadline: Jan 31 2010 Reward: $20,000 USD The Seeker is looking for methods to optimize sub-cellular localization tags for protein expression.... Methods to Analyze Consumer Emotions Deadline: Jan 17 2010 Reward: $10,000 USD The Seeker is looking for methods to analyze consumer emotions. This Challenge requires only a writ... More Challenges Powered by: nature jobs Senior Faculty Positions Torrey Pines Institute for Molecular Studies Port St. Lucie, FL Laboratory Technician (Pharmaceutics) Alliance Institute of Advanced Pharmacy and Health Sciences Hyderabad 500038 India More science jobs Post a job for free Figures & Tables See also: Research News by Jovin Export citation Search buyers guide:   ADVERTISEMENT

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