Journal home > Archive > Table of Contents > Research >  Article > Abstract

Journal home Advance online publication Current issue Archive Press releases Supplements Focuses Conferences Guide to authors Online submission Permissions For referees Free online issue Contact the journal Subscribe Advertising work@npg naturereprints About this site For librarians   NPG Resources Bioentrepreneur Nature Reviews Drug Discovery Nature Nature Medicine Nature Genetics Nature Reviews Genetics Nature Methods Nature Chemical Biology news@nature.com Clinical Pharmacology & Therapeutics Nature Conferences NPG Subject areas Biotechnology Cancer Chemistry Clinical Medicine Dentistry Development Drug Discovery Earth Sciences Evolution & Ecology Genetics Immunology Materials Science Medical Research Microbiology Molecular Cell Biology Neuroscience Pharmacology Physics Browse all publications Article Nature Biotechnology  20, 467 - 472 (2002) doi:10.1038/nbt0502-467 Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo Thi My Anh Neildez-Nguyen1, Henri Wajcman2, Michael C. Marden3, Morad Bensidhoum4, Vincent Moncollin1, Marie-Catherine Giarratana1, Ladan Kobari1, Dominique Thierry4 & Luc Douay1, 5 1  Institut National de la Santé et de la Recherche Médicale (INSERM) U417, Hôpital Saint-Antoine, Paris, France. 2  INSERM U468, Hôpital Henri Mondor, 94010 Créteil, France. 3  INSERM U473, Hôpital de Bicêtre, 94276 Le Kremlin-Bicêtre, France. 4  Institut de Protection et de Sûreté Nucléaire, DPHD, SARAM, IPSN, 92265 Fontenay-aux-Roses, France. 5  Service d'Hématologie Biologique, Hôpital Armand Trousseau, Paris, France. Correspondence should be addressed to Luc Douay luc.douay@trs.ap-hop-paris.frNew sources of red blood cells (RBCs) would improve the transfusion capacity of blood centers. Our objective was to generate cells for transfusion by inducing a massive proliferation of hematopoietic stem and progenitor cells, followed by terminal erythroid differentiation. We describe here a procedure for amplifying hematopoietic stem cells (HSCs) from human cord blood (CB) by the sequential application of specific combinations of growth factors in a serum-free culture medium. The procedure allowed the ex vivo expansion of CD34+ progenitor and stem cells into a pure erythroid precursor population. When injected into nonobese diabetic, severe combined immunodeficient (NOD/SCID) mice, the erythroid cells were capable of proliferation and terminal differentiation into mature enucleated RBCs. The approach may eventually be useful in clinical transfusion applications. MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated REFERENCE Globin Synthesis Nature Encyclopaedia of Life Sciences REVIEWS The anemia of vitamin A deficiency: epidemiology and pathogenesis European Journal of Clinical Nutrition Reviews (03 Apr 2002) RESEARCH Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1) Leukemia Original Article (01 Jun 2003) Cobblestone area-forming cells, long-term culture-initiating cells and NOD/SCID repopulating cells in human neonatal blood: a comparison with umbilical cord blood Bone Marrow Transplantation Original Article (01 Nov 2002) Anti-A isoagglutinin as a risk factor for the development of pure red cell aplasia after major ABO-incompatible allogeneic bone marrow transplantation Bone Marrow Transplantation Original Article (01 Feb 2000)  See all 7 matches for Research  Top Abstract Previous | Next Table of contents Full text Download PDF Send to a friend Save this link Open Innovation Challenges Methods of Modeling Adaptation in Populations Deadline: Dec 30 2009 Reward: $15,000 USD The analysis of adaptation with a population is a frequently encountered computational modeling scen... Single-cell Analysis Platform Deadline: Dec 02 2009 Reward: $5,000 USD This Challenge is looking for novel approaches to analyzing changes at a single-cell level. This is... More Challenges Powered by: nature jobs Group Leader Positions IMP Vienna Austria Materials Engineer Praj Matrix - Praj Industries Ltd Pune, Maharashtra Pune-411021 India More science jobs Post a job for free Figures & Tables Export citation Search buyers guide:   ADVERTISEMENT

ISSN: 1087-0156 EISSN: 1546-1696 Journal home | Advance online publication | Current issue | Archive | Press releases | Supplements | Focuses | Conferences | For authors | Online submission | Permissions | For referees | Free online issue | About the journal | Contact the journal | Subscribe | Advertising | work@npg | naturereprints | About this site | For librarians

©2002 Nature Publishing Group | Privacy policy