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Figure 4

Nature Biotechnology  20, 1140 - 1145 (2002)
Published online: 7 October 2002; | doi:10.1038/nbt747

Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and big gamma-glutamylcysteine synthetase expression

Om Parkash Dhankher, Yujing Li, Barry P. Rosen, Jin Shi, David Salt, Julie F. Senecoff, Nupur A. Sashti & Richard B. Meagher
 
Fig 4 full size
Figure 4. Relative growth inhibition and arsenic speciation of ArsC-overexpressing plants.
(A) Comparative growth inhibition of three SRS1p/ArsC lines (SRS1p/ArsC2, SRS1p/ArsC7, and SRS1p/ArsC9) and the wild-type plants grown on the indicated concentrations (the average and s.e. values of three replicates of 30 seedlings for each line). (B) The percentage relative concentration of free AsO4 3-, AsO3 3-, and As(III) tris-glutathione in leaf samples as determined by XANES at the Stanford Synchrotron Radiation Laboratory (SSRL) following the methods described by Pickering et al 9. Leaf tissues of wild-type plants and the four SRS1p/ArsC Arabidopsis lines 2, 7, 8, and 9 grown for three weeks on 75 muM arsenate were examined.

 
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