 | Figure 4
Nature Biotechnology
20, 1140 - 1145 (2002)
Published online: 7 October 2002; | doi:10.1038/nbt747
Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and -glutamylcysteine synthetase expressionOm Parkash Dhankher, Yujing Li, Barry P. Rosen, Jin Shi, David Salt, Julie F. Senecoff, Nupur A. Sashti
& Richard B. Meagher | | | | Figure 4. Relative growth inhibition and arsenic speciation of ArsC-overexpressing plants. (A) Comparative growth inhibition of three SRS1p/ArsC lines (SRS1p/ArsC2, SRS1p/ArsC7, and SRS1p/ArsC9) and the wild-type plants grown on the indicated concentrations (the average and s.e. values of three replicates of 30 seedlings for each line). (B) The percentage relative concentration of free AsO4
3-, AsO3
3-, and As(III) tris-glutathione in leaf samples as determined by XANES at the Stanford Synchrotron Radiation Laboratory (SSRL) following the methods described by Pickering et al
9. Leaf tissues of wild-type plants and the four SRS1p/ArsC Arabidopsis lines 2, 7, 8, and 9 grown for three weeks on 75 M arsenate were examined.
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