 | Figure 3
Nature Biotechnology
20, 1140 - 1145 (2002)
Published online: 7 October 2002; | doi:10.1038/nbt747
Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and  -glutamylcysteine synthetase expressionOm Parkash Dhankher, Yujing Li, Barry P. Rosen, Jin Shi, David Salt, Julie F. Senecoff, Nupur A. Sashti
& Richard B. Meagher | | | | Figure 3. Arsenic-sensitive phenotype of ArsC-expressing Arabidopsis.
(A) Leaf-specific expression of SRS1p/GUS reporter fusion (A3 and A4) is compared to GUS-stained wild type (WT: A1 and A2), confirming the strong aboveground light-induced expression from the rubisco SRS1 promoter in two-week-old plants. Stained enlarged roots (inset box) from WT and SRS1p/GUS plants are shown in panels A2 and A4, respectively. (B, C) ArsC-expressing Arabidopsis are hypersensitive to arsenate in comparison with wild type. (B) Arsenate sensitivity of the transgenic line SRS1p/ArsC9 expressing ArsC from the SRS1p promoter compared with wild type (WT) on increasing concentrations of sodium arsenate (0, 75, and 150  M) in 0.5 MS medium. (C) Enlargement of wild-type and transgenic line SRS1p/ArsC9 grown vertically on 150 M arsenate. Plants in (B) and (C) were grown for three weeks on half-strength MS medium with the arsenic concentrations indicated.
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