 | Figure 2
Nature Biotechnology
20, 1035 - 1039 (2002)
Published online: 23 September 2002; | doi:10.1038/nbt743
Engineering hypervirulence in a mycoherbicidal fungus for efficient weed controlZiva Amsellem, Barry A. Cohen
& Jonathan Gressel | | | | Figure 2. Greater efficiency of NEP1 Colletotrichum inoculum in controlling Abutilon theophrasti seedlings treated at the cotyledonary stage of development.
(A) The plants were sprayed with 5  105 chopped viable mycelial propagules/ml of water to runoff, kept 24 h at 100% humidity, and photographed two days after inoculation. (B) Development of disease symptoms following treatment. Disease ratings: 0, no effect; 1, some damage; 2, severe damage; 3, dead. Open symbols, wild type; closed symbols, strain T-20 NEP1 Colletotrichum. The plants were sprayed with  , 0.9 106,  , 2.7 106, and  , 8 106 propagules/ml, sprayed to runoff, and kept 24 h at 100% humidity. 106 propagules/ml sprayed to runoff represent  8,000 propagules/cm2 of leaf surface. Standard error bars are shown where larger than symbols. Treatments different from each other at P < 0.05 (t-test for whole period) are denoted with different letters.
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